Ocular surface reconstruction, amniotic membrane, and cultivated epithelial cells from the limbus

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Amniotic Membrane Transplantation for Ocular Surface Reconstruction

The use of amniotic membrane (or amnion) for transplantation as graft in ocular surface reconstruction is reviewed. This technique has become widespread because of the availability of the amnion, convenience and ease of use, and high and reproducible success rates. The mechanisms of action of the transplantation are varied and include the prolongation and clonogenic maintenance of epithelial pr...

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Amniotic membrane transplantation for ocular surface reconstruction.

AIMS To evaluate the efficacy of amniotic membrane transplantation (AMT) for ocular surface reconstruction. METHODS 10 consecutive patients who underwent AMT were included. The indications were: group A, cases with persistent epithelial defect after corneal abscess (n = 1), radiation (n = 1), or chemical burn (n = 3); group B, cases with epithelial defect and severe stromal thinning and impen...

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Amniotic Membrane Transplantation for Ocular Surface Reconstruction

The purpose of this study is to analyze the clinical experience and the effect of human amniotic membrane transplantation on pterygium excision and bullous keratopathy. From January 1999 to January 2001 at University Hospital »Sestre milosrdnice« amniotic membrane transplantation was performed consecutively in 21 eyes: 11 eyes with bullous keratopathy and 10 with recurrent pterygia. In the grou...

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Culture and characterization of oral mucosal epithelial cells on human amniotic membrane for ocular surface reconstruction

PURPOSE To culture oral mucosal epithelial cells on deepithelialized human amniotic membrane without the use of feeder cells and to compare the characteristics of cultured oral cells with cultured limbal and conjunctival epithelial cells for use in ocular surface reconstruction. METHODS Oral biopsies were obtained from healthy volunteers after informed consent and were cultured on deepithelia...

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Efficacy of cultivated corneal epithelial stem cells for ocular surface reconstruction

PURPOSE To investigate the clinical outcomes of cultivated corneal limbal epithelial transplantation (CLET) using human amniotic membrane for corneal limbal stem-cell deficiency. METHODS Prospective, noncomparative case series. Eighteen patients (19 eyes) with severe ocular surface diseases were chosen to undergo CLET using human amniotic membrane. Twelve eyes received auto-CLET, and seven ey...

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ژورنال

عنوان ژورنال: British Journal of Ophthalmology

سال: 2003

ISSN: 0007-1161

DOI: 10.1136/bjo.87.12.1437-a